In Vitro & In Vivo Toxicology
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Performed in-house in a quality controlled way
The Pharmacelsus service portfolio on In Vitro and In Vivo Toxicology service offers you a complete workflow. De-risk either your early stage drug discovery or late stage development programs with us. In Vitro Toxicology assays are performed in-house in a quality controlled way. In Vivo Toxicology studies according to national and international guidelines are available on rodent and non-rodent species in cooperation with our trusted partner. In Vivo non-GLP rodent studies are performed in house.
In Vitro Toxicology
Cytotoxicity
- Test Systems Established Cell Lines and Primary Cells, 2D–Monolayer, Suspension and 3D-Organoid Cultures
- Analysis
Fluorescence, Luminescence, Absorbance
- Indicator
Resazurin, XTT, MTT, ATP, LDH
- Readout
Viability, Cell Membrane Damage, Energy Content
Mitochondrial Toxicity
- Test Systems
HepG2, HepG2-Oxphos
- Analysis
Fluorescence, Luminescence
- Indicator
Resazurin, XTT, ATP
- Readout
Viability
- Format
96-Well Plate/Plate Reader
- Multiplexing of Analytical Endpoints Available
Metabolite Toxicity
- Test Systems
HepG2 w/ and w/o Metabolic Activation - by S9-Fraction
- Analysis
Luminescence
- Indicator
ATP
- Format
96-Well Plate/Plate Reader
- Metabolite Identification via LC-MS
- Reactive Metabolite Trapping in Liver Microsomes
Immunotoxicity
- Test Systems
PMBC, Monocytes, Blood
- Analysis
Luminex Technology
- Readout
Lymphocyte Subsets, Cytokine Levels
- Format
96-Well Plate/Plate Reader, Luminex
- Can be Combined with Other Systems e.g. Viability Assays
- Recommended assays according to ICH S8
Hepatotoxicity
- Test Systems
HepG2, Primary Hepatocytes
- Analysis
Fluorescence, Luminescence, Luminex - Technology
- Indicator
Resazurin, ATP
- Readout
Viability, Energy Content, Biomarker Levels
- Format
96-Well Plate/Plate Reader, Luminex
Allergic Response / Histamine Release
- Test Systems
Peritoneal Mastocytes
- Analysis
Absorption
- Indicator
O-Phtaldialdehyde
- Readout
Histamine Release
- Format
96-Well Plate/Plate Reader
In Vivo Toxicology*
Species
- Rodents
- Non-Rodent Species (please contact us)
Routes of Administration
- Oral
- Intraperitoneal
- Intravenous
- Subcutanous
- Dermal
- Rectal
- Others (please contact us)
Acute Toxicity
- Systemic Toxicity
- Phototoxicity
- Local Tolerance
- Skin Sensitisation and Irritation
- Others (please contact us)
Subchronic & Chronic Toxicity
- Rodents and Non-Rodents
- DRF Studies
- MTD Studies
- Toxicokinetics
Reproductive Toxicity
- Fertility and Reproduction
Pathology
- Histology
- Immuno-Histopathology
- Others (please contact us)
Further toxicological assays are available on request.
Please contact us for your specific toxicology study.
For In Vitro Toxicology
Dr. Julia Susewind
In Vitro Toxicology
susewind@pharmacelsus.de
For In Vivo Toxicology
Anika Christoffel
In Vivo Pharmacology & Pharmacokinetics
christoffel@pharmacelsus.de