Your Challenges Require Smart Solutions:

In Vitro ADME

Professionalism and flexibility

The in vitro ADME team of Pharmacelsus combines professionalism of the pharmaceutical industry with the flexibility to adapt standard assays for your specific challenges. We use a consultative approach to set-up tailor-made assays together with you. Studies are conducted to meet regulatory requirements.

Evaluate the “druggability” of your compounds by the following set of in vitro ADME assays.

Physico-Chemical Parameters

Chemical Stability
Aqueous Solubility

Stability in Biological Matrices

Blood
Plasma
Intestinal Fluids
Further Matrices on Request
All Preclinical Species Available

Membrane Permeability

PAMPA
Artificial BBB
Bidirectional Caco2

Interaction with Drug Transporters*

ABC-ATP Binding
SLC Transporters:
Inhibition/Uptake
PgP Interaction

*in cooperation

Protein Binding Mechanism

PPB (Crossfiltration or Rapid Equilibrium Dialysis)
SPB (Crossfiltration or Rapid Equilibrium Dialysis)
Blood Partitioning
Microsomal Binding

Brain Tissue Binding

Rat, Mice Tissue
Ultrafiltration
Rapid Equilibrium Dialysis

Metabolic Stability

Liver or Intestinal Microsomes
Liver or Intestinal S9 Fraction
Hepatocytes
All Preclinical Species Available

Reactive Metabolite Trapping

Liver Microsomes
GSH

Metabolite Identification

Liver or Intestinal Microsomes
Liver or Intestinal S9 fraction
Hepatocytes
Metabolic Pathway

CYP Inhibition

Recombinant Human CYPs
IC₅₀ Determination
Human Liver Microsomes*
Mode of Inhibition, Inhibition Kinetics

*according to EMA/FDA Guidelines

CYP Induction

Hepa RG®
Human Hepatocytes
Functional Activity
mRNA

CYP Phenotyping

Recombinant Human CYPs
Loss of Parent Compound
Clint
Half-Life
Formation of Major Phase 1 Metabolites and Metabolic Pathway Elucidation

Please contact us for your specific ADME study.

MUDr. Maria Li
 Director Business Development
li@pharmacelsus.de

Dr. Julia Przibilla 
ADMET & In Vitro Pharmacology 
przibilla@pharmacelsus.de